GC-MS Determination of Bioactive Constituents of Hydrilla verticillata (L.f.) Royle. Collected from Unpolluted and Polluted Water Sources

The investigation was carried out to determine the chemical components of H. verticillata using Perkin-Elmer Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC/MS analysis of ethanol extract of H. verticillata revealed the existence of five compounds in samples collected from polluted water and five compounds in samples collected from unpolluted water. Out of ten compounds, sesquiterpene compound (Coryan-17-ol, 18,19-di dehydro-10-methoxy-acetate), Steroid compound (Ergost -5-en-ol, 22, 23-dimethyl acetate), plasticizer compound (1,2 Benzene dicarboxylic acid butyl octylester), Linoleic compound (10-Octadecenoic acid, methyl ester), Stearic acid (Pentadecanoic acid, 14-methyl, methyl ester) and Phytol (Diterpene compound) have anti-fungal, anti-bacterial, anti-arthritic, anti-inflammatory, anti-cancer, anti-oxidant, anti-diabetic and enhances the immunity. The results of this study offer a platform of using H. verticillata as herbal alternative for various diseases.