In-silico and In-vitro Antimitotic Activity of Some Novel 6-Fluoro-1, 2, 4-Triazolo-Benzothiazole Analogues

Worldwide, cancer is a leading cause of death in both developed and developing countries. Numerous natural and manmade anticancer drugs treat solid tumors, lymphomas, and leukemias in different forms. In this work, nine 6-fluoro-triazolo-benzothiazole derivatives were prepared and evaluated for in vitro antimitotic activity. In addition, an in-silico study was also done using tubulin protein (PDB: 6QQN) by molecular docking method. Thin-layer chromatography (TLC) was used to monitor the progress of the reaction progress. Results revealed that TZ2 and TZ9 were the most active compounds with antimitotic action opposing the standard drug, aspirin. Results of molecular docking exhibited the inhibitory potential of triazole-benzothiazole against tubulin protein. The mitotic study indicates the efficacy of triazole-benzothiazole analogues in inhibiting the proliferation of cancer cells either by promoting microtubule formation or affecting microtubules, thereby preventing microtubule breakdown. The obtained results suggest that germinated mung beans may be a useful tool for quickly and affordably evaluating new medicines' cytotoxic effects.