CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM MARINE ACTINOMYCETES ANTAGONISTIC TO URINARY TRACT BACTERIAL PATHOGENS

The development of multi drug resistant uropathogens is a big threat to human race. Infections with multidrug- resistant bacteria are hard to treat. The present study Characterization of bioactive compounds from marine actinomycetes antagonistic to urinary tract bacterial pathogens is aimed to prove that marine actinomycetes have some bioactive compounds which are antagonistic to multi drug resistant uropathogens. In this study there were 14 different urine samples which were collected from urinary tract infection (UTI) suspected patients and from these specimens five uropathogens were isolated. Thirty seven (37) marine actinomycetes were isolated from various marine samples that were collected from different stations of Kovalam coastal region, part of Arabian Sea on the western coast of India. The isolated colonies were studied on their morphological characteristics. These diverse colonies were observed and which indicated the potential diversity of the actinomycetes that had been isolated from various sources. The antagonistic activity studies showed that among the 37 marine actinomycetes isolates 10 isolates showed significant antagonism against all the five test organisms that were cultured including Escherichia coli, Klebsiella sp., Pseudomonas sp., Enterococcus sp., Proteus sp. in the primary screening. The isolate KVSD9 showed maximum inhibitory activity against Escherichia coli (18mm). The result was consistent on both well diffusion and disc diffusion methods. Similarly KVSD4 showed maximum activity against Klebsiella sp. KVSD35 showed maximum activity against Pseudomonas sp. in well diffusion assay. But in disc diffusion method KVSD2 was the most potent strain against Pseudomonas sp. Against Enterococcus sp. KVSD9 showed the highest antagonistic activity in both the secondary assays followed by KVSD2. KVSD4 was the most potent isolate against Proteus sp. in both disc and well diffusion methods.