ANTI-INFLAMMATORY ACTIVITY OF TITANIUM DIOXIDE NANOPARTICLES SYNTHESISED USING GRAPE SEED EXTRACT: AN in vitro STUDY

Background: Titanium dioxide nanoparticles (TiO2 NPs) synthesized from plant sources are eco-friendly, cost effective and have a wide range of applications. The anti-inflammatory property of titanium dioxide produced by various herbal sources has well been studied. Grape seeds have been used in this study to produce TiO2 NPs due to their anti-inflammatory, anti-diabetic, anti-cholesterol properties.

Aim: The aim of this study was to determine the anti-inflammatory activity of titanium dioxide nanoparticles synthesised using grape seed extract.

Materials and Methods: Preparation of grape seed extract, synthesis of titanium dioxide nanoparticles using grape seed extract in Lark refrigerated centrifuge, preparation of nanoparticles powder, characterisation using UV-Vis Spectroscopy and study of anti-inflammatory activity using Bovine serum albumin using Diclofenac sodium as control.

Results: The peak found in spectroscopy indicated the formation of titanium dioxide nanoparticles. The anti-inflammatory action of grape seed mediated TiO2 NPs increases with an increase in the concentration from 10 μl, 20 μl, 30 μl, 40 μl to 50 μl.

Conclusion: Titanium dioxide nanoparticles produced using grape seed extract have good anti-inflammatory activity and can be used in the future as pastes, gels etc. for topical application instead of using conventional anti-inflammatory agents to minimize their side effects.