A STUDY ON PLASMID MEDIATED AmpC BETA-LACTAMASE AND ITS ANTIMICROBIAL SUSCEPTIBILITY PATTERN AMONG ESCHERICHIA COLI AND Klebsiella Species

This is a descriptive study done during the period of May 2013 to August2014 at Department of Microbiology, Bharath Institute of Higher Education and Research. A total of 100 isolates (E. coli n=57 and Klebsiella spp. n=43) were processed in the present study. Out of these, 41(41%) were from urine sample, followed by17 (17%) pus samples, 25(25%) sputum samples, 9(9%) Blood samples, 6(6%) stool samples and 2(2%) samples were from pleural fluid and Ascitic fluid each. Out of these 100 isolates 33 strains were from ICU, 44strains were from wards and 23 strains were from outpatients. ESBL production was seen in 41(41%) strains among the 100 isolates, by CLSI decried criteria. The prevalence of Amp C beta-lactamases in found tube 16% by multiplex PCR. The most predominant geneis CIT followed by DHA. The rate of Amp C beta-lactamas production was found to relatively high in hospitalized patients. In the present study all the Amp ‘·; ta-lactamase showed 100% susceptibility to Meropenem both in E. coli and Klebsiellas pp. All Amp C producers were resistant to cephalosporins except cefepime, which is a fourth generation cephalosporin.