PHYSICOCHEMICAL AND MICROBIOLOGICAL RESULTS OF HYPERTHERMAL (HOT) MINERAL WATER IN RUPITE, BULGARIA AS MODEL SYSTEM FOR ORIGIN OF LIFE

The objective of current research is to prove the presence of bacteria genus Bacillus in hot mineral water from Rupite, Bulgaria. The water of Rupite is rich in hydrocarbon and calcium ions. The presence of bivalent Calcium (Ca2+) and Magnesium (Mg2+) ions in warm and hot mineral waters take part in the activation of cortex-lytic enzymes during germination of Bacillus subtilis (Igura, 2003).

In 2010 Ignatov and Mosin indicate the possibility for origin of life and living matter in hot mineral water 3.5 billion years ago. Comparative analyses are conducted based on experiments for the similarities of spectra of hot mineral waters and cactus juice. The closest matches are the spectra of cactus juice and the water from Rupite, Bulgaria. Such compatibility is not observed between the cactus juice and sea water. In the cactus juice and the hot mineral water we observed five comparable local extremums (8.95; 9.67; 9.81; 10.47; 11.13 μm), whereas there was only one (9.10 μm) with the sea water. It is therefore justified to suggest that most probably life originated in hot mineral water. According to their temperature they can be cold (up to 37 °С), warm (from 37°C to 60 °C) and hot (over 60 °C). This is temperature standard in Bulgaria, European Union.